milliplex maptm 32-mouse-plex cytokine detection system  (Millipore)

Journal: Mediators of Inflammation

Article Title: Flavonoid Naringenin: A Potential Immunomodulator for Chlamydia trachomatis Inflammation

doi: 10.1155/2013/102457

Figure Lengend Snippet: Naringenin downregulates inflammatory mediators in C. trachomatis infected mouse J774 macrophages. Macrophages (10 6 cells/mL) were seeded in 24-well plates and were either infected with live C. trachomatis (10 5 IFU/well) or LPS at 1 μ g/mL. After 2-day infection, naringenin at 0.01 to 10 μ g/mL was added to cell cultures and the production levels of cytokines (a) and chemokines (b) were quantified in supernatants collected 2 days later employing multiplex ELISA. ***indicates significant difference ( P < 0.001) between C. trachomatis treated cells and those treated with various concentrations of naringenin using the two-tailed unpaired Student's t -test. Each bar represents the average of samples run in duplicates and the data are representative of three separate experiments.

Article Snippet: Milliplex mouse 32-plex cytokine and chemokines detection reagent (catalogue number MPXMCYTO-70 K-PMX32) was purchased from Millipore (EMD Millipore Corporation, Billerica, MA, USA) and the assay was performed as described [ ].

Techniques: Infection, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: PLoS ONE

Article Title: Different Patterns of Expression and of IL-10 Modulation of Inflammatory Mediators from Macrophages of Lyme Disease-Resistant and -Susceptible Mice

doi: 10.1371/journal.pone.0043860

Figure Lengend Snippet: BMDM were stimulated with live Bb or L-OspA in the presence or absence of exogenous or α-IL-10 (or its isotype control) Ab as described in and . Cell-free supernatants were harvested from cultures at 24 h and protein determinations were made by multiplex ELISA. Fold decrease (A) was calculated as stimulant/stimulant exposed to exogenous IL-10. Fold increase (B) was calculated as stimulant + α IL-10 Ab/stimulant+isotype control Ab. A one-way ANOVA followed by a Bonferroni post-hoc test in conjugation with two tailed Mann Whitney test was used for data analyses. Significance was established at P <0.001 = ***, P <0.01 = ** and P <0.05 = *. Each symbol represents fold change for a specific cytokine or chemokine. Each fold change is representative of two independent experiments.

Article Snippet: Concentrations of inflammatory mediators were quantified in cell-free supernatants of BMDM cultures using the Milliplex mouse 32-plex cytokine and chemokine detection system (Millipore Corporation, Bedford, MA) according to the manufacturer's instructions.

Techniques: Control, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Conjugation Assay, Two Tailed Test, MANN-WHITNEY